It's Lupin's greatest challenge yet as he goes toe to toe with the mysterious and deadly inhabitants of an island that doesn't exist on any map – the Island of Assassins.
The "Tarantulas" are the most fearsome gang of assassins on the face of the planet. But, in order to solve a dark mystery from his past, Lupin must lower himself into the heart of the spider's lair! Can Lupin fight an assassin army, steal their fabled cache of gold, find a cure for an "incurable" poison, and get the girl, all at the same time? One thing is certain, with Jigen, Goemon, and Fujiko along for the ride; it's going to be nonstop adventure as the gang tries to escape the Island of Assassins!